• JUI CHAKRABORTY

Articles written in Bulletin of Materials Science

• One-pot synthesis of CaAl-layered double hydroxide–methotrexate nanohybrid for anticancer application

One-pot (co-precipitation) synthesis route was employed for the first time to synthesize pristine CaAl-layereddouble hydroxide (LDH) and in-situ intercalation of the anticancer drug methotrexate (MTX) to prepare CaAl-LDH–MTXnanohybrid. An increase in the interplanar spacing of the (003) plane from 8.6 $\AA$ in pristine CaAl-LDH bilayered structureto 18.26 $\AA$ in CaAl-LDH–MTX nanohybrid indicated successful intercalation of anionic MTX into the interlayer space ofCaAl-LDH. This was supported by the transmission electron micrographs, which showed an increase in average interlayerspacing from 8.7 $\AA$ in pristine LDH to 18.31 $\AA$ in LDH–MTX nanohybrid. Particle size and morphology analysis of pristineCaAl-LDH and LDH–MTX nanohybrid using both dynamic light scattering (DLS) technique and transmission electronmicroscopy (TEM) indicated a decrease in average particle size in LDH–MTX nanohybrid as compared with that of pristineLDH. Thermogravimetric analyses (TGA) revealed an enhancement in decomposition temperature of MTX bound to CaAl-LDH nanohybrid to 380$^{\circ}$C as compared with 290$^{\circ}$C in pure MTX molecule, indicating enhanced thermal stability, which supports stable electrostatic interaction of MTX within the interlayer position of LDH. CHN (carbon hydrogen nitrogen) analysis revealed nearly 49 wt% of MTX loading into CaAl-LDH, which closely matched with the result obtained from TGA of the nanohybrid. Cumulative release of MTX from CaAl-LDH–MTX in phosphate buffer solution showed a non-lineardependence with incubation time. Release mechanism of MTX from LDH–MTX nanohybrid was governed by diffusionmechanism at physiological pH of 7.4. The in vitro cytotoxicity study of LDH–MTX nanohybrid using MG-63 humanosteosarcoma cell line indicated enhanced inhibition of the cancer cell proliferation compared with the MTX drug alone.

• An in vitro comparative study of layered-double hydroxide nanoconjugate in the delivery of small interference and short-hairpin ribonucleic acid

Alzheimer’s disease is a disease which cannot be cured completely. In this aspect ribonucleic acid interference(RNAi) therapy is a prospective therapeutic mechanism which can be used for identifying a future curative procedure. RNAitherapy comprises small interfering RNA (siRNA), short hairpin (shRNA) and micro-RNA therapeutics. Within these threemechanisms we have identified two of them as an effective method of combating this genetic incurable disease. siRNAs andshRNAs are very much effective in vitro that is already proved in many research work. In our study we have used a very potent, biocompatible nanoparticle-layered double hydroxide for delivering these macromolecules. However, the intercalation and cellular internalization of these macromolecules demonstrated significant differences. As siRNAs have low-molecular weight than shRNAs they demonstrated different characteristics in the case of internalization within layered-double hydroxide and while cellular internalization. At the end of this study it has been found that both of these macromolecules may be used as a therapeutic approach of Alzheimer’s disease after studying it in future in animal and human subjects.

• Bulletin of Materials Science

Volume 45, 2022
All articles
Continuous Article Publishing mode

• Dr Shanti Swarup Bhatnagar for Science and Technology

Posted on October 12, 2020

Prof. Subi Jacob George — Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bengaluru
Chemical Sciences 2020