• Amalesh Choudhury

      Articles written in Proceedings – Animal Sciences

    • Growth ofAcanthamoeba culbertsoni (Singh and Das 1970) andAcanthamoeba rhysodes (Singh 1952) (Protozoa: Gymnamoebia) in cultures

      Mrinal Ghosh Amalesh Choudhury

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      Six strains of amoebae belonging to two species, vizAcunthamoeba culbertsoni andAcanthamoeba rhysodes, were isolated during the 3 prominent seasonal periods of premonsoon, monsoon and postmonsoon from the south of Sagar Island facing Bay of Bengal (21°56′ to 21°88′ N and 88°08′ to 88°16′E). The actual sampling sites were the lowest low tide belt areas. They were co-cultured in pairs, for respective seasonal periods, in 20‰ saline distilled water agar medium with their habitat associated microbiota as food. Dominance of all the seasonal isolates ofAcanthamoeba culbertsoni over the corresponding strains ofAcanthamoeba rhysodes arising out of competitive growth has been observed. Thus in laboratory maintained cultural conditions, strains ofAcanthamoeba culbertsoni show remarkable adaptive superiority over those ofAcanthamoeba rhysodes.

    • Aspects of culture:Acanthamoeba astronyxis (Ray and Hayes 1954) from Bay of Bengal coasts, India

      Mrinal Ghosh Amalesh Choudhury

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      Acanthamoeba astronyxis strains, isolated from intertidal belts of Sagar Island (21°30′ to 21°50′ N and 88°04′ to 88°08′ E) facing Bay of Bengal, were cultured in saline distilled water agar medium supplemented withEscherichia coli as food bacterium. Population growth in bay water agar, cerophyl bay water agar and soil extract distilled water agar were compared with special reference to devised saline distilled water agar medium. The overall growth showed little variation over ranges of media salinity (5–30‰). However, greater values were encountered in media of intermediate range. Isolate from mangrove litter-soil of highest high tide belt (strains) grew more efficiently in cerophyl bay water agar and soil extract distilled water agar media while mid-littoral and lowest low tide isolates preferred bay water agar and saline distilled water agar media more.

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