Volume 72, Issue 3
September 1970, pages 99-138
pp 99-114 September 1970
Experiments were undertaken to standardise the techniques involved in large-scale cultivation of ergot [Claviceps purpurea (Fr.) Tul.]. It was found that the rye crop planted from the last week of September up-to the middle of October to be more suitable for obtaining a good yield of ergot. The spore suspension of the ergot fungus raised on wheat or rye grain medium retained its infective ability even after storing for about 3 days at room temperature (20±2° C.) and for about 5 days when stored under refrigerated conditions (5°C.). Among the four synthetic liquid media tested the medium described by Amiciet al. (1967) proved to be more suitable for raising the ergot inoculum on a large scale and it also retained its infectivity even after diluting twenty times of its volume. Rye plots, if inoculated two times by the needle board puncture method with the inoculum containing about 41,000 spores/mm.3 was found to be sufficient to yield maximum quantity of ergot sclerotia. The yield of the ergot sclerotia obtained from the plots inoculated by the needle board puncture method was found to be almost two times the yield obtained from those plots which were inoculated by spray method. Variation in alkaloid content has been observed in the ergot sclerotia obtained from the same strain and also within the same sclerotium. Experiments carried out on the probability of contamination of the ergot infection to the other cereal crops indicated that there are very little chances of ergot infection spreading to the other cereal crops and also the over-summering of the ergot sclerotia in the field under the natural conditions of the Jammu region.
pp 115-119 September 1970
The accidental discovery of a cell with haploid number of diplochromosomes in squashes of colchicine-treated root tips led to a search for haploid anaphases in treated roots allowed to recover in water. No haploid anaphases were seen. Apart from the divisional stages of diploid and tetraploid nuclei, cells with two pro-, meta- and ana-phases were observed. The formation of distinct cell boundaries by each nucleus of an originally multinucleate cell indicates their potentialities in this direction.
pp 120-128 September 1970
pp 129-138 September 1970
Transeliminase activity of culture filtrates ofPhytophthora rubra was detected spectrophotometrically. Reaction products with pectin absorbed maximally at 230 mµ at pH 8·5. Ca++ ions stimulated this activity. TBA test confirmed TE activity by showing maximum absorption at 550 mµ at pH 7·6. This indicated the presence of pectin transeliminase (PTE) in the culture filtrate. Products of reaction mixture with Napolypectate gave maximum absorption at 227 mµ at pH 9·4. This also indicated the presence of polygalacturonate transeliminase (PGTE) in the culture filtrate.