Volume 71, Issue 4
April 1970, pages 133-176
pp 133-144 April 1970
Five populations ofImpatiens species from disturbed habitats of Simla Hills were studied. Associations of six morphological characters was analyzed. Using the metroglyph analysis procedure, the presence of hybrid swarms with hybridization and introgression betweenI. racemosa, I. laxiflora andI. scabrida was postulated to explain the cause of the plant-to-plant variability in the several characters observed in these populations.
pp 145-149 April 1970
In the anterior region of the larval body and above the brain, there are present imaginal sacs called the paired frontal sacs. Each sac is distinguished into an anterior antennal disc and a posterior eye disc. During the pupal development these sacs develop into the antennae and the eyes respectively of the imago. The developmental changes occurring in the eye disc of the pupae of different ages are described here in detail. The eyes are completely developed when the imago emerges from the puparium.
pp 150-156 April 1970
The adrenal glands of a total of 153 C3H (Jax) virgin mice were studied. The experimental groups consisted of intact, sterile-mated or mammectomised females with or without DMB treatment. The histologically determinable age changes in the adrenal glands of the C3H (Jax) strain were few and not distinctive. The pleomorphy of the “A” type cells was remarkable. The degeneration of the lower zona fasciculata found in C3H (Jax) adult females has not been previously described in the literature. Significant alterations were not produced in the adrenal histology by repeated pseudopregnancies or by total mammectomy. Since in the control groups as well as in the carcinogen-treated groups considerable individual variations existed, it was difficult to assess the significance of quantitative changes in the “A” cells, in the degenerating cells of the lower zona fasciculata, or in vascularity and proliferation of the zona reticularis. DMB produced adrenal tumors, but pre-tumorous changes were not seen in the adrenal glands which were non-tumorous or which had a microscopical tumor in a part of the gland.
pp 157-163 April 1970
DMB induced tumors in the adrenal glands of intact, sterile-mated or mammectomised C3H (Jax) female mice. Fewer tumors developed when a small single dose of DMB was given while the female was pseudopregnant. The tumors occurred in 10–30% of the mice and involved one or both adrenals. They were microscopical or large encapsulated growths of upto 10 mm. in size and were transplantable.
The tumors started as tiny nodules in the subcapsular region, expanding and replacing the entire adrenal gland. They were undifferentiated, consisting of cells with dark spindle or plump ovoid nuclei with chromatin granules. The cytoplasm was scanty. Mitoses were few. There was abundant reticulum in the stroma. Evidence is presented to show that these hitherto undescribed tumors in DMB-treated animals originate from the altered subcapsular “A” type mesenchymal cell.
pp 164-170 April 1970
An undifferentiated adrenal tumor from a DMB-treated C3H (Jax) female mouse was found transplantable in about 50% male and 75% female homologous animals used. The “takes” were better and faster in the females; the sex in which the tumor had originated. The latent period shortened with successive passages and was shorter for females. Histologically, the transplanted tumors resembled the original tumor but tended to be more pleomorphic, highly vascular, and showed increased mitotic activity. Evidence was found to show that testosterone which inhibits induction of adrenal tumors cannot prevent their proliferation.
pp 171-176 April 1970
The influence of component monosaccharide sugars on thein vitro synthesis of oligosaccharides by the three fungi,viz., Macrophoma allahabadensis Kapoor and Tandon,Curvularia tuberculata Jain andDrechslera australiense (Bugn.) Subram. and Jain, has been studied. The former two organisms were isolated from the diseased fruits of guava and the last from tomato. The present investigations have shown that the persistence of oligosaccharides could be altered by extra supply of the component sugars in the medium. When maltose was supplied at the rate of 10 g./litre in the medium an oligosaccharide I (Rf 0·18) was synthesized by all the three organisms. An addition of 5 g. glucose to a maltose medium induced the synthesis of an additional oligosaccharide II (Rf 0·30). Thus oligosaccharide (Rf 0·30) was produced only in the culture filtrate ofMacrophoma allahabadensis andCurvularia tuberculata. The addition of D-glucose or D-fructose to a sucrose medium showed no effect on the synthesis of oligosaccharide. The nature of synthesized oligosaccharides was studied partially after subjecting them to total and partial hydrolysis and results have been recorded.