Volume 52, Issue 1
July 1960, pages 1-31
pp 1-8 July 1960
1. Three crystalline compounds A, B and C have been isolated from human smegma.
2. Chemistry of compound A has been studied in detail. It has been shown to be an ester ofβ-cholestanol with a saturated fatty acid of the formula C20H40O2. Compound C has been identified with an authentic sample ofβ-cholestanol by mixed melting point and infra-red spectrum. Compound B occurs in very small amounts. Available evidence indicates it to be a hydroxy steroid.
3. Three new ester ofβ-cholestanol were prepared in connection with the work.
The study was carried out under the auspices of the Indian Council of Medical Research. The authors are grateful to the organisation for the help and encouragement received from it. The authors should also like to thank Drs. R. V. Rajam, T. V. Patel, Varma, P. N. Shaha and M. L. Shroff for a generous collection of human smegma from the hospitals.
pp 9-18 July 1960
1. Growth characteristics ofTrametes cingulata Berk. have been studied in culture under different environmental conditions.
2. Basidiospores from freshly taken fructifications have been found to germinate very quickly on agar medium under ordinary conditions of diffused light and temperature (30° C.) of the laboratory. Basidiospores always germinate by means of a single germ-tube.
3. Being a ‘white rot’ fungus, it gives positive reaction to Bavendamm’s oxidase test.
4. The fungus has been grown on 2·5%malt-agar medium with seven different grades of pH, three on either side of the neutral. The best growth has been found in the medium having neutralpH value. An interesting feature is the wide range of tolerance of the fungus to both acidic and alkaline conditions to a certain extent.
5. Cultural characteristics of the fungus have been studied on four different media under a set of constant conditions. Best growth has been obtained onpotato-dextrose-agar, lesser inmalt-agar, thenCzapeck’s synthetic-agar andwood-decoction-agar following in succession.
pp 19-26 July 1960
By taking the amount of CO2 produced as an index of respiratory activity outside water, the air-breathing function of the following species has been demonstrated:
O. punctatus, which possesses paired pharyngeal diverticula as organs of breathing air, produces the least quantity of CO2. InC. batrachus andH. fossilis the production of CO2 is relatively higher. Both these species possess opercular lungs. The differences in the CO2 produced between the three species fall in close agreement with the structural complexities of their accessory respiratory organs.
The rate of production of CO2 in all the three species decreases in relation to time. After 24 hours most of the fishes become moribund but soon recover when kept in aerated water.
pp 27-31 July 1960
The mature leaves ofManilkara hexandra exhibit sclerenchymatous idioblasts in close proximity to major veins and veinlets. It is found that the sclerenchymatous initials are transformed spongy cells near the differentiating procambial strands.