Volume 51, Issue 5
May 1960, pages 205-248
pp 205-210 May 1960
The nucleus with its limiting membrane and organelles was visible in the majority of the yeast cells stained vitally with the fluorochrome, acridine orange, at a dilution of 1 in 40,000. The intra-nuclear structures could be distinguished by their differential fluorescence. The chromocenters were green while the nucleolar equivalents were orange. The vacuole showed no fluorescence.
pp 211-218 May 1960
1. It has been shown that the common Indian cattle leech,Hirudinaria granulosa is capable of surviving indefinitely in a salinity of 12·96‰ if acclimatized gradually to increasing media of salinity.
2. The maximum survival concentration lies between 30% and 40% sea-water (salinity 32·4‰).
3.Hirudinaria granulosa loses weight when transferred to dilutions of sea-water. In media of lower salinity it was found that after an initial rapid loss of weight the leeches gradually reached a steady level and this weight was maintained. Return to freshwater did not result in complete recovery of the original weight.
4. The influence of salinity on oxygen consumption was studied. It was noted that the maximum oxygen consumption was in 25% sea-water while the minimum was in 100% sea-water.
pp 219-226 May 1960
The rate of hydrolysis of protein during flue-curing was examined in the light of a relationship between protein and soluble nitrogen in the leaves at the time of harvest.
It was shown that a high ratio of protein N to (soluble N—nicotine N) is associated with a rapid rate of proteolysis and a low ratio with a slow rate.
pp 227-241 May 1960
pp 242-248 May 1960
The failure of pea (Pisum sativum) to grow in ‘goradu’ soil of Central Gujarat without artificial inoculation of the seed with the specific root nodule bacteria has been investigated. The study of a large number of soil fungi isolated from ‘goradu’ soils failed to show that they had any selective antibiotic activity against the pea rhizobia. It has been explained that the high sand content of this soil and the lower thermal death point of the pea rhizobia are the probable causes for the failure. The high sand content does not give any protection to the pea rhizobia against the high temperatures of the summer months with the result that their number gets so reduced and are not able to nodulate plants sufficiently.