Volume 50, Issue 6
December 1959, pages 305-359
pp 305-318 December 1959
pp 319-339 December 1959
pp 340-348 December 1959
1. Attempts have been made to induce hepatic lesions in albino rats by varying the quantity of the protein (casein) and by withholding the supply of the vitamins of the B2 complex.
2. On the vitamin B2 complex deficient diets, the average life-span of the rats was largely influenced by the casein content of the diet, while those which received similar casein diets with yeast as supplement lived longer.
3. Fatty infiltration of the liver with progressive fibrosis (akin to human portal cirrhosis) can be more easily produced in rats by prolonged feeding of diets low in protein (casein) content and deficient in the vitamins of the B2 complex.
4. Increasing the level of protein (casein) in the diet or supplementing low protein diets with yeast has a marked corrective effect on fatty infiltration of the liver and this is attributed to their respective methionine and choline content.
5. In general, the vitamins of the B2 complex have a marked beneficial effect on the fatty livers when given early enough as a supplement.
pp 349-359 December 1959
In an attempt to study the microbial spoilage of canned food seven bacteria were isolated from seven different spoiled canned foods collected from various sources. When the pure cultures of the bacterial isolates were inoculated into freshly prepared and sterilized cans of curried vegetable, sliced potato and mixed fruit, all of them proved to cause harmful effects to a lesser or greater extent.
The bacteria were studied in detail for their morphological, cultural and physiological properties including their capacity to utilize certain carbon and nitrogen sources and on the basis of the results obtained they are identified asBacillus circulans Jordan,B. brevis Migula,B. subtilis Cohn,B. coagulans Hammer,B. licheniformis (Weighmann) Chester,Lactobacillus fermenti Beijerinck andClostridium histolyticum (Weinberg and Seguin) Bergeyet al.