An effective method for purifying apiin from parsley seeds is to convert it into the lead salt and recover it by decomposition with hydrogen sulphide. It then crystallises readily from alcohol and yields a crystalline acetate. The work on the complete methylation and hydrolysis has been revised using the pure sample and also purifying the methylated glycoside obtained as intermediate. The resulting 5:4′-o-dimethyl apigenin is found to melt correctly at 297–8° as mentioned by Bakeret al. Even the impure sample obtained earlier from crude apiin can be purified through its acetate, and then its melting point is found to be higher as mentioned above.
Extraction of the parsley plant grown in Delhi yielded only apiin as the anthoxanthin component and the presence of luteolin glycoside reported by von Gerichten could not be detected.