An essential point which has not been established in a simple manner by the previous study of pedicinin and its synthesis relates to the position of the methoxyl group. This has now been settled in an unambiguous manner by the reduction of pedicinin with stannous chloride to dihydropedicinin and its ethylation. The product is found to be identical with 4-methoxy-2∶3∶5∶6-tetraethoxy chalkone obtained synthetically starting from 2∶6 dimethoxy quinol. This chalkone undergoes oxidative de-ethylation with nitric acid yielding ethyl pedicinin and eventually pedicinin.
The toxic properties of dimethyl pedicinin (2∶4∶5-trimethoxy-quino chalkone) have been studied using fish.