Analysis and study of acid hydrolysis show that hibiscitrin is a monoglucoside of hibiscetin. It undergoes complete methylation with dimethyl sulphate and anhydrous potassium carbonate in anhydrous acetone medium; on hydrolysing the methyl ether a O-hexamethyl hibiscetin is obtained which yields trimethyl gallic acid on fission with alkali. From this reaction and from other characteristic properties of the above partial methyl ether it is concluded that the free hydroxyl is in the 3-position and that hibiscitrin is a 3-glucoside.