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      https://www.ias.ac.in/article/fulltext/pram/065/05/0967-0972

    • Keywords

       

      Molecular motors; atomic force microscope (AFM); immobilization; biomolecular electronics and nanotechnology

    • Abstract

       

      The present study reports the covalent immobilization of myosin on glass surface andin-vitro motility of actin-myosin biomolecular motor. Myosin was immobilized on poly-L-lysine coated glass using heterobifunctional cross linker EDC and characterized by AFM. Thein-vitro motility of actin was carried out on the immobilized myosin. It was observed that velocity of actin over myosin increases with increasing actin concentration (0.4–1.0 mg/ml) and was found in the range of 0.40–3.25 μm/s. The motility of actinmyosin motor on artificial surfaces is of immense importance for developing nanodevices for healthcare and engineering applications

    • Author Affiliations

       

      Ellis Bagga1 Sunita Kumari1 Rajesh Kumar1 Rakesh Kumar1 R P Bajpai1 Lalit M Bharadwaj1

      1. Biomolecular Electronics and Nanotechnology Division, Central Scientific Instrument Organisation, Sector 30/C, Chandigarh - 160 030, India
    • Dates

       
  • Pramana – Journal of Physics | News

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      Posted on July 25, 2019

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