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      Permanent link:
      https://www.ias.ac.in/article/fulltext/plnt/099/04/0307-0312

    • Keywords

       

      Solanum sarrachoides Sendt.; histogenesis; micropropagation; organogenesis

    • Abstract

       

      In vitro direct induction of multiple shoot buds ofSolanum sarrachoides Sendt. was obtained from leaf segments using Murashige and Skoog’s basal medium supplemented with 6-γ-γ-(dimethylallylamino) purine (1·5–2·5 mgl−1). Maximum shoot bud proliferation was observed in the medium containing 2 mgl−1 and better growth of plants with well defined roots, leaves, flowers and fruits in the subsequent stages of development. Histological examination at different stages of development confirmed that the multiple buds produced on excised leaf segments arise adventitiously through the formation of numerous tiny protuberances. The complete sequence of events leading to multiple bud formation based on histological studies has been presented.

    • Author Affiliations

       

      N Banerjee1 S Mukhopadhyay1 A K Sharma1

      1. Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Calcutta - 700 019, India
    • Dates

       
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