Shoots originating from nodal stem explants ofSalix babylonica showed maximum proliferation through axillary branching on a modified Murashige-Skoog medium supplemented with 1 mg/l of benzylaminopurine. Incorporation of naphthaleneacetic acid or gibberellic acid did not enhance shoot proliferation in the presence of benzylaminopurine. Shoots were grown to a suitable length on a medium with low concentration of benzylaminopurine. Subsequently they were excised and allowed to root on a basal medium, with or without a short pre-treatment with naphthaleneacetic acid. Rooted plantlets were transferred to pots and maintained under high humidity conditions to prevent their desiccation. The high humidity conditions were gradually withdrawn and the plantlets established as independently growing saplings.