The effect of various factors uponin vitro germination ofAmaryllis vittata pollen was studied to find out optimum cultural requirements for obtaining high percentage of pollen germination and maximum tube growth. Temperature below −10°C was suitable for the storage ofAmaryllis pollen. Maximum germination was observed in sucrose medium. Pollen germination and the tube growth were maximal at the culture density of 5 mg/ml of the culture medium. The optimum temperature was 28±2°C. Studies on respiration suggested that optimum conditions for maximum O2 uptake were the same as for optimum pollen germination and pollen tube growth rate.