• Effect of buffer and pH on growth and protein content of carrot (Daucus carota L.) in liquid shake culture

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    • Keywords


      Tissue culture; buffer; carrot; pH

    • Abstract


      Phosphate, succinate, acetate and maleate buffers were tested for buffering B5 liquid medium. Phosphate (0·02 M) and acetate (0·002 M) were the most suitable buffers as they promoted dry weight accumulation and nitrogen assimilation, they also exhibited less change in pH after 5 days cultivation of carrot cells. The pH increase from 5·5 up to 6·5 also increased the dry weight accumulation and cell size; pH 7·0 was detrimental to dry weight accumulation.

    • Author Affiliations


      R P Singh1 B D Singh1 R B Singh1 2

      1. Department of Genetics and Plant Breeding, Banaras Hindu University, Varanasi - 221 005, India
      2. Food and Agriculture Organisation RAFE, Maliwan Mansion, Phra Atit Road, Bangkok, Thailand
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