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      Permanent link:
      https://www.ias.ac.in/article/fulltext/plnt/087/10/0261-0269

    • Keywords

       

      Azadirachta indica (neem) gum; polysaccharide composition; glucosaminyl asparginyl bond

    • Abstract

       

      Azadirachta indica (neem) exudate gum was treated with pronase for 48 h followed by chromatography on TEAE-cellulose and the major polysaccharide was isolated. The polysaccharide covalently associated with remnant protein, was homogeneous as indicated by rechromatography on TEAE-cellulose, paper electrophoresis, gel chromatography under dissociating conditions on Bio-Gel P-l00 and P-300. The monosaccharide units, galactose, arabinose, glucuronic acid, fucose and glucosamine were present in a molar ratio of 86 ∶ 70 ∶ 30 ∶ 10 ∶ 1. Thirteen amino acids constituted the protein portion. The linkage between the polysaccharide and the protein was a glucosaminyl asparginyl bond. Limited hydrolysis showed that fucose and arabinose were at the non-reducing ends of the polysaccharide and galactose and glucuronic acid were in the central core.

    • Author Affiliations

       

      B Ramakrishna Nayak1 Radhakrishna Rao1 T N Pattabiraman1

      1. Department of Biochemistry, Kasturba Medical College, Manipal - 576 119
    • Dates

       
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