• Growth of and trehalase activity in the thermophilic fungusThermomyces lanuginosus

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      https://www.ias.ac.in/article/fulltext/plnt/087/10/0231-0241

    • Keywords

       

      Thermomyces lanuginosus ; thermophilic fungus; trehalase; sporulation; catabolite repression

    • Abstract

       

      The thermophilic fungus,Thermomyces lanuginosus, was grown in a glucose-asparagine liquid medium. Optimal mycelial growth occurred at 50°C. The conditions for sporulation were different from those required for vegetative growth. the former being favoured by lower nitrogen level and temperature. Trehalase (α, α-glu coside-l-glucohydrolase, EC 3.2.1.28) was one of the most active glycosidases at 50°C. Non-sporulating mycelium had higher levels of this enzyme than the sporulating mycelium. Trehalase was synthesized constitutively and its activity appears to be controlled by catabolite repression.

    • Author Affiliations

       

      A R S Prasad1 Ramesh Maheshwari1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012
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