• Nucleotide sequence of 5S RNA ofMycobacterium smegmatis

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      Permanent link:
      https://www.ias.ac.in/article/fulltext/plnt/087/10/0213-0224

    • Keywords

       

      RNA sequence; comparison of sequences; thin layer electrophoresis; M values; Mycobacterium smegmatis ; 5S RNA

    • Abstract

       

      32P labelled 5S RNA isolated fromMycobacterium smegmatis was digested withT1 and pancreatic ribonucleases separately and fingerprinted by two dimensional high voltage electrophoresis on thin-layer DEAE-cellulose plates. The radioactive spots were sequenced and their molar yields were determined. The chain length of the 5S RNA was found to be 120. It showed resemblances to both prokaryotic and eukaryotic 5S RNAs.

    • Author Affiliations

       

      P Jagadeeswaran1 Joseph D Cherayil1

      1. Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012
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