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    • Keywords


      Leaf clearing; trichloroacetic acid; phenol; fungal staining

    • Abstract


      Clearing can be successfully attained by soaking portions of leaves in a mixture of trichloroacetic acid and phenol (2:1) for 10–15 min at 60° C. These can be stained with writing ink (Chelpark make). The intensity of staining does not change even in permanent preparations. The preparations made as outlined above have been found specially beneficial for studying the morphology of fungi parasitic on leaves.

    • Author Affiliations


      H Y Mohan Ram1 Vijay Laxmi Nayyar1

      1. Department of Botany, University of Delhi, Delhi 110 007
    • Dates


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