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      Permanent link:
      https://www.ias.ac.in/article/fulltext/plnt/087/01/0063-0066

    • Keywords

       

      Triticale ; haemagglutinin; animal erythrocytes; blood group specificity

    • Abstract

       

      Saline extracts of several varieties ofTriticale had haemagglutinin activity against rabbit, rat and fowl erythrocytes. In contrast to the wheat germ lectin theTriticale lectin was inactive against human B, 0 blood group type erythrocytes and rather high concentrations of the lectin are needed to agglutinate human A blood group type erythrocytes. TheTriticale lectin was purified about 20-fold with a 10% recovery of activity from one of the varieties (DTS 138) by (NH4)2SO4 fractionation followed sequentially by chromatography on DEAE-cellulose and sulphopropyl-Sephadex. Approximately 4 μg of the purified lectin caused visible agglutination with trypsinised rabbit erythrocytes. Among a variety of sugars tested D-glucose, D-mannose and N-acetyl-D-glucosamine (2·5-7·5mM) caused inhibition of agglutination.

    • Author Affiliations

       

      K Hariharan1 D Rajagopal Rao1

      1. Discipline of Biochemistry and Applied Nutrition, Central Food Technological Research Institute, Mysore - 570013
    • Dates

       
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