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      https://www.ias.ac.in/article/fulltext/jgen/101/0029

    • Keywords

       

      protein tyrosine phosphatase receptor type J; cervical cancer; tumour-suppressor gene.

    • Abstract

       

      Squamous cell carcinoma of the uterine cervix (CSCC) is one of the leading causes of death in Indian women. Protein tyrosine phosphatase receptor (PTPR) type J (also known as DEP1) is a recently reported tumour suppressor receptor phosphatase. Critical molecular analysis of PTPRJ/DEP1 (11p11.2) has not performed in CSCC to date. Here, we observed frequent downregulation of cancer samples (n=31) at the transcriptional level. Immunohistochemistry revealed concordant low expression of PTPRJ protein with a few samples showing intermediate expression. To probe for the cause of such downregulation of the gene in CSCC (n=155), we analysed the copy number and promoter methylation of PTPRJ. The genetic locus showed deletion (14.8%) and the promoter showed methylation (33.5%) of PTPRJ. To the best of our knowledge, for the first time we explored the molecular status of PTPRJ although we observed no statistically significant association with the prognosis of Indian CSCC patients (n=76). However, we observed enhanced expression ofPTPRJ protein levels that contributes to effective cisplatin chemotherapy in the SiHa cell line. Thus, the present study paves the way for further research into the plausible mechanisms of downregulation of PTPRJ in cervical cancer.

    • Author Affiliations

       

      ANIRBAN ROYCHOWDHURY1 2 MUKTA BASU1 DEBOLINA PAL1 PRIYANKA DUTTA1 SUDIP SAMADDER1 MONDAL3 ANUP KUMAR ROY4 SUSANTA ROYCHOUDHURY5 CHINMAY KUMAR PANDA1

      1. Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata 700 026, India
      2. Present Address: Division of Hematology, Oncology, and Palliative Care, Department of Internal Medicine, Massey Cancer Center, Virginia Commonwealth University, Richmond, USA
      3. Department of Gynecology Oncology, Chittaranjan National Cancer Institute, Kolkata 700 026, India
      4. Department of Pathology, Nil Ratan Sircar Medical College and Hospital, Kolkata 700 014, India
      5. CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India
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