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      https://www.ias.ac.in/article/fulltext/jgen/100/0038

    • Keywords

       

      next-generation sequencing; genotyping-by-sequencing; skim sequencing; marker; genome.

    • Abstract

       

      High-throughput genotyping has become more convenient and cost-effective due to recent advancements in next-generation sequencing (NGS) techniques. Numerous approaches exploring sequencing advances for genotyping have been developed over the past decade, which includes different variants of genotyping-by-sequencing (GBS), and restriction-site associated DNA sequencing (RAD-seq). Most of these methods are based on the reduced representation of the genome, which ultimately reduces the cost of sequencing by many folds. However, continuously lowering the cost of sequencing makes it more convenient to use whole genome-based approaches. In this regard, skim sequencing, where low coverage whole-genome sequencing is used for the identification of large numbers of polymorphic markers cost-effectively. In the present review, we have discussed recent technological advancements, applicability, and challenges of skim sequencing-based genotypic approaches for crop improvement programmes. Skim sequencing is being extensively used for genotyping indiverse plant species and has a wide range of applications, particularly in quantitative trait loci (QTL) mapping, genomewide association studies (GWAS), fine genetic map construction, and identification of recombination and gene conversion events in various breeding programmes. The cost-effectiveness, simplicity, and genomewide coverage will increase the application of skims sequencing-based genotyping. The article summarizes the protocol, uses, bioinformatics tools, its application, and future prospects of skim sequencing in crop improvement.

    • Author Affiliations

       

      PARDEEP KUMAR1 MUKESH CHOUDHARY1 B. S. JAT1 BHUPENDER KUMAR1 VISHAL SINGH1 VIRENDER KUMAR2 DEEPAK SINGLA3 SUJAY RAKSHIT1

      1. ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana 141 004, India
      2. National Agri-food Biotechnology Institute (NABI), Mohali 140 308, India
      3. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004, India
    • Dates

       
  • Journal of Genetics | News

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