Cloning and expression of AGL19 gene in two Lonicera macranthoides varieties
YU QING LONG XIA LIU JUAN ZENG CAN LI XIANG DAN LIU RI BAO ZHOU
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AGLl9 is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The fulllengthcDNAs of AGL19in conventional Lonicera macranthoides (Lm-AGL19) and the mutant ‘Xianglei’ cultivar (Lm-XL-AGL19) wereobtained using rapid amplification of cDNA ends and the expression vectors for Lm-XL-AGL19were constructed to investigate the roles of AGL19 in the 'Xianglei' cultivar. Lm-AGL19 (GenBank: MK419948) and Lm-XL-AGL19 (GenBank: MK419948) were isolated from theconventional variety and 'Xianglei' cultivar of L. macranthoides, respectively. Lm-AGL19 is 1274 bp in length, whereas Lm-XL-AGL19 is 1264-bp long, and both include a 654 bp open reading frame, encoding 217 amino acids, which has a highly conserved MADS_MEF2_likedomain and a moderately conserved K-box domain, belonging to the type II MADS-box family of genes. Quantitative real-time polymerasechain reaction indicated that the expression levels of these genes at different flowering stages were significantly different, and that the geneswere also expressed in stems and leaves. Lm-XL-AGL19 is underexpressed at flowering period 5 that the key time node for corollaexpansion and nonexpansion, while LM-AGL19 is overexpressed during this flowering period. AGL19 was speculated to be a functionalgene causing different phenotypes in the two L. macranthoides varieties. The successfully constructed plant expression vector provides anexperimental reference for further research on the function of this gene and the basis for the excellent phenotype of L. macranthoides 'Xianglei'.
YU QING LONG1 2 XIA LIU1 2 JUAN ZENG1 2 CAN LI1 2 XIANG DAN LIU1 2 3 RI BAO ZHOU1 2
Volume 102, 2023
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