• BADH1 is associated with fragrance in sorghum (Sorghum bicolor (L.) Moench) cultivar ‘Ambemohor’

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      https://www.ias.ac.in/article/fulltext/jgen/100/0003

    • Keywords

       

      Sorghum; fragrance; aroma; BADH1 gene; Ambemohor.

    • Abstract

       

      Aroma is an important trait that can enhance the product value in several crops. Pandan-like fragrance resulting fromaccumulation of 2-acetyl-1-pyrroline (2AP) is one of the pleasant aromas in food crops which is caused by null or missense mutations inbetaine aldehyde dehydrogenase 2 (BADH2) gene. In addition, betaine aldehyde aehydrogenase 1 (BADH1) has shown to be associatedwith aroma in rice. In this study, we investigated the genetics controlling coconut juice-like fragrance in inflorescence of sorghum cultivar ‘Ambemohor’. 2AP analysis in seeds revealed that Ambemohor possessed no 2AP. An F$_{2}$ population developed from the cross betweenAmbemohor $\times$ KU630 (nonfragrant) segregated into a ratio of 3 (fragrant) : 1 (nonfragrant), suggesting that the coconut juice-like fragrancein Ambemohor is controlled by a single dominant gene, designated ‘(Aro(’. Bulked segregant analysis suggested that the gene controllingfragrance in Ambemohor is located on sorghum chromosome 6. Quantitative trait locus (QTL) analysis identified a major QTL, (qAro6.1,for the fragrance located on chromosome 6 between markers SB3567 and SB3570. Bioinformatics analysis revealed that SB3567 andSB3570 were 217.8 kb apart and there were 29 annotated genes in this region including BADH1. Sequence analysis revealed that BADH1sequences in Ambemohor and KU630 differed in size, but their coding sequences (CDS) were of same size. CDS alignment revealed foursingle-nucleotide polymorphisms (SNP$_{s}$) between Ambemohor and KU630 in which two SNP$_{s}$ caused amino change in BADH1 of Ambemohor. These results suggested that BADH1 is a candidate gene for the coconut juice-like fragrance in Ambemohor.

    • Author Affiliations

       

      THRAIPOB MONKHAN1 CHEN XIN2 PRAKIT SOMTA1 3 4

      1. Faculty of Agriculture at Kamphaeng Saen, Department of Agronomy, Kasetsart University, Nakhon Pathom 73140, Thailand
      2. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People’s Republic of China
      3. Center of Advanced Studies for Agriculture and Food (CASAF), Kasetsart University Institute for Advanced Studies, Kasetsart University (NRU-KU), Bangkok 10900, Thailand
      4. Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand
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