Fusarium stalk rot disease (FSR) of maize caused by Fusarium verticillioides (Sacc.) Nirenberg is becoming an importantbiotic production constraint in many of the major maize growing areas causing substantial yield losses. Inbreds are preferred as parents inhybrid development owing to homozygous nature and high heterotic ability. Double haploid (DH) technology has emerged as a significantmilestone. A total of 339 DH lines were generated from two inbred lines, VL1043 (susceptible) and CM212 (resistant), through in vivohaploid induction method. The 339 DH lines along with parents were phenotyped for their response to the FSR at the College ofAgriculture, V. C. Farm, Mandya, India during summer, kharif and rabi seasons of the 2019–2020. Best linear unbiased predictors (BLUPs)were estimated for the FSR disease scores over three seasons. Awide range of BLUP scores of three to nine indicated the presence of highervariation for response of DH lines to FSR disease. The higher estimates of standardized range (1.31) and phenotypic coefficient of variation(19.80) also displayed higher variability. Nine lines were moderately resistant and 188 exhibited moderately susceptible reaction. Thedistribution of DH lines was positively skewed (1.34) and platykurtic (2.31) which suggested complementary epistasis and involvement oflarge number of genes in the disease expression.