QTL identification for downy mildew resistance in cucumber using genetic linkage map based on SSR markers
PAWINEE INNARK HADSAYA PANYANITIKOON CHANULAK KHANOBDEE SOMPID SAMIPAK CHATCHAWAN JANTASURIYARAT
Click here to view fulltext PDF
Permanent link:
https://www.ias.ac.in/article/fulltext/jgen/099/0081
Fourteen cucumber lines were tested for genetic homozygosity and performed pairwise comparison to identify a pair with thehighest DNA polymorphic level. Cucumber accessions CSL0067 and CSL0139 were selected to generate 315 F2 populations. The geneticlinkage map based on 66 polymorphic SSR markers was constructed. It composed of eight linkage groups (LGs) spanning 474.4 cM.Downy mildew disease reaction was evaluated in cotyledons, first and second true leaf on 7, 10, and 14 day after inoculation. The resultsshowed that downy mildew resistance was controlled by multiple recessive genes. The susceptible to resistant ratio of F2 progenies fit 9:7 susceptible/resistant segregation types corresponding to duplicate recessive epistasis. Fourteen QTLs were detected. The phenotypicvariance ranged from 5.0 to 12.5%, while LOD values ranged from 3.538 to 9.165. Two major QTLs and two QTL hotspots were identified.Moreover, the additive effects data explained that these QTL reduced downy mildew susceptibility.
PAWINEE INNARK1 HADSAYA PANYANITIKOON2 CHANULAK KHANOBDEE3 SOMPID SAMIPAK2 4 CHATCHAWAN JANTASURIYARAT2 4
Volume 99, 2020
All articles
Continuous Article Publishing mode
Click here for Editorial Note on CAP Mode
© 2021-2022 Indian Academy of Sciences, Bengaluru.