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https://www.ias.ac.in/article/fulltext/jgen/099/0045

avirulence gene; AVR-Pita1 gene; genetic variation; resistant gene.

Rice blast disease, caused by Magnaporthe oryzae, is one of the most importance diseases of rice production worldwide. The keyrole of defense mechanism to combat this fungus in rice follows the gene-for-gene concept, which a plant resistant (R) gene product recognizes a fungal avirulent (AVR) effector and triggers the hypersensitive response. However, the AVR genes have been shown to be rapidly evolving resulting in high level of genetic diversity. The aims of this study were to examine the nucleotide sequence variation of AVR-Pita1 gene in Thai rice blast isolates and to identify the severity of blast disease using isogenic line of Pita gene. Seventy-six rice blast isolates collected from different parts of Thailand were used. Gene specific primers for AVR-Pita1 gene coding sequence were designed and used for identifying the genetic diversity of AVR-Pita1 gene by PCR amplification and sequencing. The obtained sequences were analysed for genetic variation and genetic relationship. Our results revealed the association between the sequence variations of AVR-Pita1 and selective forces from Pita gene. This phenomenon demonstrated the coevolution between rice blast resistant gene in rice and avirulent gene in blast fungus. The information about variation and evolutionary mechanisms of AVR gene obtained from this study can be used in rice blast resistant breeding programme.

KATANYUTITA DAMCHUAY¹ APINYA LONGYA¹ TANEE SRIWONGCHAI² PATTAVIPHA SONGKUMARN³ NONGLAK PARINTHAWONG⁴ KULCHANA DARWELL⁵ SUCHEELA TALUMPHAI⁶ PIYAMA TASANASUWAN⁷ CHATCHAWAN JANTASURIYARAT⁸

1. Faculty of Science, Department of Genetics, Kasetsart University, Chatuchak, Bangkok 10900, Thailand
2. Faculty of Agriculture, Department of Agronomy, Kasetsart University, Chatuchak, Bangkok 10900, Thailand
3. Faculty of Agriculture, Department of Plant Pathology, Kasetsart University, Chatuchak, Bangkok 10900, Thailand
4. Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
5. Phrae Rice Research Center, 98 Moo 5 Mae Khum Mee, Mueang, Phrae 54000, Thailand
6. Faculty of Liberal Arts and Science, Department of Science and Technology, Roi Et Rajabhat University, Roi Et 45120, Thailand
7. Faculty of Science, Department of Zoology, Kasetsart University, Chatuchak, Bangkok 10900, Thailand
8. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart (University CASTNAR, NRU-KU), Kasetsart University, Bangkok 10900, Thailand

Published

27 May 2020

Manuscript received

31 October 2019

Manuscript revised

9 February 2020

Accepted

24 February 2020