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paternal lineage; Holstein Friesian crossbred bull; acrocentric Y-chromosome.

Karyotyping is one among the culling parameter used for taking up culling decisions. Cytogenetic screening of breeding bulls has been recommended to screen for chromosomal abnormalities before semen production in artificial insemination. The chromosomal analysis of a Holstein Friesian crossbred bull revealed the presence of acrocentric Y-chromosome, which was further confirmed by CBGbanding. The shape of the Y-chromosome determining that male line used for crossbreeding was from indigenous origin. Karyotyping is a best and reliable technique for the identification of crossbred calves born to the indigenous bulls.

V. HARSHINI¹ P. KUMARASAMY¹ S. M. K. KARTHICKEYAN¹ D. CAUVERI¹ A. MANGALA GOWRI² S. RANGASAMY³

1. Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai 600 007, India
2. Centre for Stem Cells and Regenerative Medicine, Madras Veterinary College, Chennai 600 007, India
3. Department of Veterinary Gynaecology and Obstetrics, Madras Veterinary College, Chennai 600 007, India

Published

7 April 2020

Manuscript received

7 January 2020

Manuscript revised

8 February 2020

Accepted

17 February 2020