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https://www.ias.ac.in/article/fulltext/jgen/099/0026

duck; retinoic acid-inducible gene I; gene expression; antivirus; poly(I:C); Anas platyrhynchos

Intracellular double-stranded RNA (dsRNA) is a chief sign of replication for many viruses. Pattern recognition receptors(PRRs) of the innate immune system detected the dsRNA and initiate the antiviral responses. Retinoic acid-inducible gene I (RIG-I), a member of PRRs, plays an essential regulatory role in dsRNA-induced signalling. In this study, the full-length complementary DNA (cDNA) of duck RIG-I (duRIG-I) was cloned using the reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE). The cDNA of duRIG-I contained 97-bp 5'UTR, 141-bp 3'-UTR and 2802 bp complete open-reading frame (ORF) encoding 933 amino acids. Multiple sequence alignments showed that duRIG-I shared high similarity with RIG-I from other vertebrates. Quantitative real-time PCR (qRT-PCR) analysis revealed that duRIG-I mRNA was expressed in all tested tissues, with high levels in the liver, heart, spleen, kidney and thymus, while lower in the duodenum. duRIG-I could be induced by treatment with poly(I:C). Further, overexpression of duRIG-I significantly activated the transcription of poly(I:C)-induced IFN-b, IRF7, TRIF, Mx, STAT1 and STAT2 mRNA, and duRIG-I knockdown showed the opposite results. Overall, our results suggested that duRIG-I could be an important receptor for mimicking antiviral state in duck, which warrant further studies to show the possible mechanism.

TIANTIAN GU^{1 2} GUOQIN LI^{2 3} YONG TIAN^{2 3} LI CHEN^{2 3} XINSHENG WU¹ TAO ZENG² QI XU¹ PYRYDONOV SVLADYSLAV⁴ GUOHONG CHEN¹ LIZHI LU²

1. iangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, Yangzhou University, Yangzhou, Jiangsu 225009, People’s Republic of China
2. nstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, People’s Republic of China
3. Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou, Zhejiang 310021, People’s Republic of China
4. National University of Life and Environment Sciences of Ukraine, Kiev 03041, Ukraine

Published

19 March 2020

Manuscript received

22 August 2019

Manuscript revised

6 January 2020

Accepted

14 January 2020