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      https://www.ias.ac.in/article/fulltext/jgen/099/0021

    • Keywords

       

      lentil; quantitative-trait loci; molecular map; early plant vigour; Lens culinaris.

    • Abstract

       

      Lentil is one of the most important food legume species, however its genetic and genomic resources remained largely uncharacterized and unexploited. In the past few years, a number of genetic maps have been constructed and marker resources have been developed in lentil. These resources could be exploited for understanding the extent and distribution of genetic variation in genus Lens andalso for developing saturated and consensus genetic maps suitable for quantitative trait loci (QTL) mapping and marker-assisted selection.The present study aims to enrich polymerase chain reaction-based linkage map of F10 recombinant inbred lines (RILs) population of 94 individuals derived from cross WA8649090 9 Precoz and identification of QTLs linked to early plant vigour traits. Of the 268 polymorphic markers (93 simple sequence repeats (SSR), three inter-simple sequence repeats (ISSRs) and 172 random amplified polymorphic DNA (RAPDs)), 265 (90 SSRs, three ISSRs and 172 RAPDs) were mapped on seven linkage groups, varying in length between 25.6 and 210.3 cM, coverage of 809.4 cM with an average marker spacing of 3.05 cM. The study also reported assigning of 24 new cross-genera SSRs of Trifolium pratense on the present linkage map. The RILs along with the parents were screened for shoot length, root length, seedling length, dry weight, number of leaves and number of branches based on two replications under polyhouse conditions. A QTLhotspot consisting of six QTLs for shoot length (cm), root length (cm) and seedling length (cm) was observed between a map distances of56.61 and 86.81 cM on LG1.

    • Author Affiliations

       

      RUSHIKESH MANE1 MEGHA KATOCH1 MANEET SINGH2 REECHA SHARMA1 T. R. SHARMA3 R. K. CHAHOTA1

      1. Department of Agricultural Biotechnology, CSK Himachal Pradesh Agriculture University, Palampur 176 062, India
      2. Department of Biotechnology, ICAR-Indian Grassland and Fodder Research Institute, Jhansi 284 003, India
      3. ICAR-Indian Institute of Agricultural Biotechnology, Garhkhatanga, Ranchi 834 010, India
    • Dates

       
  • Journal of Genetics | News

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