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      https://www.ias.ac.in/article/fulltext/jgen/099/0020

    • Keywords

       

      microsatellites; African pangolin; Temminck’s ground pangolin; Smutsiinae; Smutsia temminckii

    • Abstract

       

      Temminck’s ground pangolin (Smutsia temminckii) is one of four species of pangolin, endemic to Africa. Two of the African pangolin species are listed as vulnerable and two are listed as endangered on the International Union for Conservation of Nature Red List of Threatened Species due to their ongoing exploitation for traditional medicine and bushmeat. In this study, we developed 30 species-specific short-tandem repeats (STRs) in Temminck’s ground pangolin using next-generation sequencing. The markers were also optimized for crossamplification in other African species. All the markers amplified successfully in Temminck’s ground pangolin with allelic polymorphisms observed in 87% of the markers in giant pangolin (S. gigantea) whereas 60% of the markers were amplified polymorphic loci in both whitebellied pangolin (Phataginus tricuspis) and black-bellied pangolin (P. tetradactyla). Analysis of diversity estimates showed moderate levels of variability in Temminck’s ground pangolin (Na = 5; Ho = 0.559), giant pangolin (Na = 4.909; Ho = 0.514) and white-bellied pangolin (Na= 2.686; Ho = 0.541) with lower values being observed in black-bellied pangolin (Na = 3; Ho = 0.242). This study provides data of the first available STR markers which was amplified in all four African pangolin species that can now be used in conservation genetic and evolutionary aspects of population histories.

    • Author Affiliations

       

      ZELDA DU TOIT1 2 DESIRÉ L. DALTON1 2 MORNÉ DU PLESSIS1 3 RAYMOND JANSEN4 J. PAUL GROBLER2 ANTOINETTE KOTZÉ1 2

      1. South African National Biodiversity Institute, P.O. Box 754, Pretoria 0001, South Africa
      2. Department of Genetics, University of the Free State, P.O. Box 339, Bloemfontein 9300, South Africa
      3. Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7535, Western Cape Province, South Africa
      4. Department of Environmental, Water and Earth Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa
    • Dates

       
  • Journal of Genetics | News

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