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    • Keywords

       

      Indo-China rice; classification; DNA marker; sequence-related amplified polymorphism marker; intersimple sequence repeat marker; insertion–deletion marker.

    • Abstract

       

      Rice is believed to have originated from Indo-China, area between China and India, and then spread throughout the world. The Indochina region mainly includes countries like Thailand, Laos and Vietnam, which are the world’s major rice exporters. Rice varieties grown in this area are highly diverse due to their different environment, ecosystem and climatic conditions. The objective of this study was to evaluate the genetic relationship of Indochina rice varieties using intersimple sequence repeat (ISSR), sequence-related amplified polymorphism (SRAP) and insertion–deletion (InDel) markers. Forty-six rice varieties, including 16, 4,11 and 15 from Thailand, China, Laos and Vietnam, respectively were used in this study. Seventeen of the 20 ISSR primers showed 82.96% polymorphism. At the same time, 17 of the 30 primer pairs of SRAP marker showed clearDNA amplification, which resulted in 84.79% polymorphism. Ninety-seven of 133 InDel markers have about 99.47% polymorphism. Three markers showed average PIC score ranging from 0.20 to 0.26. When the analysis was conducted using UPGMA clustering method, it was found that the combined data from three markers gave a better result than each marker separately. The results from clustering analysis showed that all accessions can be grouped based on their location and can be categorized into two major groups. Useful results from this study could bring substantial benefits and ultimately help the rice breeders to develop elite rice varieties in future.

    • Author Affiliations

       

      PATTARABORN MOONSAP1 NUTTHALAK LAKSANAVILAT2 SITTIPUN SINUMPORN3 PIYAMA TASANASUWAN2 SUREEPORN KATE-NGAM4 CHATCHAWAN JANTASURIYARAT1 5

      1. Faculty of Science, Department of Genetics, Kasetsart University, 50 Ngam, Wong Wan Rd., Ladyaw, Chatuchak, Bangkok 10900, Thailand
      2. Faculty of Science, Department of Zoology, Kasetsart University, 50 Ngam, Wong Wan Rd., Ladyaw, Chatuchak, Bangkok 10900, Thailand
      3. Rajamangala University of Technology Isan, 744 Suranari Rd., Muang District, Nakhon Ratchasima 30000, Thailand
      4. Faculty of Agriculture, Department of Agronomy, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand
      5. Centre for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (CASTNAR, NRU-KU), Kasetsart University, Bangkok 10900, Thailand
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    • Supplementary Material

       
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