• Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jgen/098/0066

    • Keywords

       

      association mapping; biofortification; DNA markers; lentil; population structure; mixed linear model.

    • Abstract

       

      Iron (Fe) and zinc (Zn) deficiencies are wide spread in South Asia and Africa. Biofortification of food crops is a viable means of addressing micronutrient deficiencies. Lentil is an important pulse crop that provides affordable source of proteins, minerals, fibre and carbohydrates for micronutrient deficient countries. An association mapping (AM) panel of 96 diverse lentil genotypes fromIndia and Mediterranean region was evaluated for three seasons and genotyped using 80 polymorphic simple-sequence repeat (SSR) markers for identification of the markers associated with grain Fe and Zn concentrations. A Bayesian model based clustering identified five subpopulations, adequately explaining the genetic structure of the AM panel. The linkage disequilibrium (LD) analysis usingmixed linear model (MLM) identified two SSR markers, GLLC106 and GLLC108, associated with grain Fe concentration explaining 17% and 6% phenotypic variation, respectively and three SSR markers (PBALC 364, PBALC 92 and GLLC592) associated with grain Zn concentration, explaining 6%, 8% and 13% phenotypic variation, respectively. The identified SSRs exhibited consistentperformance across three seasons and have potential for utilization in lentil molecular breeding programme.

    • Author Affiliations

       

      HARISH KUMAR1 2 AKANKSHA SINGH1 3 H. K. DIKSHIT1 GYAN P. MISHRA1 M. ASKI1 MAHESH C. MEENA4 SHIV KUMAR5

      1. Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
      2. Punjab Agriculture University, RRS, Faridkot, Punjab 151 203, India
      3. Amity Institute of Organic Agriculture, Amity University, Noida 201 303, India
      4. Division of Soil Science and Agricultural Chemistry, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
      5. Biodiversity and Integrated Gene Management Program, International Centre for Agricultural Research in the Dry Areas, Rabat 10106, Morocco
    • Dates

       
    • Supplementary Material

       
  • Journal of Genetics | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2022-2023 Indian Academy of Sciences, Bengaluru.