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      https://www.ias.ac.in/article/fulltext/jgen/098/0018

    • Keywords

       

      grain protein content; S2 stage; grain filling; NAC gene; Oryza sativa.

    • Abstract

       

      In this study, we show that NAC-like transcription factor (TF) has 90% sequence similarity with cDNA of the OsNac5 gene present in the NCBI database. Phylogenetic analysis of the NAC gene family was performed with inclusion of the highly diverse C-terminal sequences. We report that this gene is also found to be orthologous to Glycine max NAC8, NAC2, Triticum aestivum NAC6 and paralogous to OsNAC6. mRNA was purified from five recombinant inbred lines (RILs) and parents of rice at three different stages of grain filling under aerobic conditions, with grain protein content (GPC) spanning from 4 to 14%. TheNAC-like TF encoding a protein was found to be upregulated at the S2 stage in the leaf (3.9-fold) and panicle (1.84-fold) of parent HPR14 and in five RILs (1.9 to 4.51-fold in leaves and 0.47 to 3.2-fold in panicles). Expression analysis of the NAC-like TF encoding a protein for the rice gene was found to be upregulated at the S2 stage in the leaf and panicle of parental line HPR14 and RILs with high protein content.

    • Author Affiliations

       

      GAURAV SHARMA1 ATUL KUMAR UPADYAY2 HANAMAREDDY BIRADAR3 SONIA4 SHAILAJA HITTALMANI3

      1. School of Agriculture, Lovely Professional University, Phagwara 144 411, India
      2. School of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144 411, India
      3. Marker Assisted Selection Laboratory, Department of Genetics and Plant Breeding, University of Agricultural Sciences, Bengaluru 560 065, India
      4. School of Pharmaceutical and Healthcare Sciences, CT University, Ludhiana 142 024, India
    • Dates

       
  • Journal of Genetics | News

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