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      https://www.ias.ac.in/article/fulltext/jgen/098/0007

    • Keywords

       

      yak; Y-chromosome; AMELX gene; AMELY gene; marker; sex determination

    • Abstract

       

      Yak, an economically important bovine species considered as lifeline of the Himalaya. Indeed, this gigantic bovine is neglected because of the scientific intervention for its conservation as well as research documentation for a long time. Amelogenin is an essential protein for tooth enamel which eutherian mammals contain two copies in both X and Y chromosome each. In bovine, the deletion of a fragment of the nucleotide sequence in Y chromosome copy of exon 6 made Amelogenin an excellent sex-specific marker. Thus, an attempt was made to use the gene as an advanced molecular marker of sexing of the yak to improve breeding strategies and reproduction. The present study confirmed that the polymerase chain reaction amplification of the Amelogenin gene with a unique primer is useful in sex identification of the yak. The test is further refined with qPCR validation by quantifying the DNA copy number of the Amelogenin gene in male and female. We observed a high level of sequence polymorphisms of AMELX and AMELY in yak considered as novel identification. These tests can be further extended into several other specialized fields includingforensics, meat production and processing, and quality control.

    • Author Affiliations

       

      P. P. DAS1 G. KRISHNAN2 J. DOLEY1 D. BHATTACHARYA3 S. M. DEB4 P. CHAKRAVARTY1 P. J. DAS5

      1. Indian Council of Agricultural Research-National Research Centre on Yak, Dirang 790 101, India
      2. Indian Council of Agricultural Research-National Institute of Animal Nutrition and Physiology, Bengaluru 560 030, India
      3. Indian Council of Agricultural Research-Central Island Agricultural Research Institute, Garacharama, Port Blair 744 101, India
      4. Indian Council of Agricultural Research-National Dairy Research Institute, Karnal 132 001, India
      5. Indian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati 781 131, India
    • Dates

       
  • Journal of Genetics | News

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