• Development and characterization of 33 microsatellite loci for the tiger frog Hoplobatrachus rugulosus (Wiegmann 1834) through transcriptome sequencing

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    • Keywords


      Dicroglossidae; genetic diversity; microsatellite; transcriptome; Hoplobatrachus rugulosus.

    • Abstract


      The tiger frog Hoplobatrachus rugulosus (Wiegmann 1834) is a large robust dicroglossid frog widely distributed in southern China, Malaysia, Myanmar, Vietnam and Thailand. The escaped bred tiger frog introduced from Thailand hybridized with Chinese native population may have affected the genetic diversity of local Chinese tiger frogs. However, previous microsatellite loci of this species do not offer enough information to construct the genetic map. Here, we reported 33 new microsatellite loci from transcriptome sequencing for H. rugulosus. Alleles ranged between 1 and 10 per locus and only one locus (HRT001) was monomorphic. The polymorphic information content, observed and expected heterozygosity were 0–0.794, 0–0.969 and 0–0.831, respectively. None of the loci was observed in linkage disequilibrium and two loci (HRT023 and HRT068) deviated from Hardy–Weinberg equilibrium after Bonferroni correction for multiple tests. These transcriptome-derived microsatellite markers will be usedto study the genetic divergence and construct the genetic map in H. rugulosus.

    • Author Affiliations



      1. College of Ecology, Lishui University, Lishui 323000, Zhejiang, People’s Republic of China
      2. College of Animal Science and Technology, Zhejiang A&F University, Lin’an 311300, Zhejiang, People’s Republic of China
      3. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, People’s Republic of China
    • Dates

  • Journal of Genetics | News

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