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      https://www.ias.ac.in/article/fulltext/jgen/097/05/1339-1351

    • Keywords

       

      genetic resources; ex situ conservation; wild selections; microsatellites; simple sequence repeats; consensus genotypes.

    • Abstract

       

      The American cranberry (Vaccinium macrocarpon) is an endemic domesticated species that has become an economically important commercial fruit crop. The USDA-ARS National Clonal Germplasm Repository (NCGR) houses the national Vaccinium collection, which includes representatives of historical cranberry cultivars and wild-selected germplasm. The objective of this study wasto examine the genotypes of 271 cranberry plants from 77 accessions representing 66 named cultivars using 12 simple-sequence repeats to assess clonal purity and cultivar relatedness. Using principal components analysis and neighbour-joining based on estimated genetic distances between individuals, we identified 64 unique genotypes and observed that intracultivar variants (i.e. subclones) existed in the germplasm collection and in the commercial bogs where some accessions originated. Finally, through a comparison of the genotypes of this study with the previous studies, pedigree analysis and the study of the geographic distribution of cranberry diversity, we identified consensus genotypes for many accessions and cultivars. We highlight the important role that the NCGR collection playsfor ex situ conservation of cranberry germplasm for future breeders and researchers. The NCGR continues to search for historically relevant cultivars absent from the collection in an effort to preserve these genotypes before they are lost and no longer commercially grown.

    • Author Affiliations

       

      B. SCHLAUTMAN1 2 G. COVARRUBIAS-PAZARAN2 L. RODRIGUEZ-BONILLA2 K. HUMMER3 N. BASSIL3 T. SMITH4 J. ZALAPA2 5

      1. The Land Institute, 2440 E. Water Well Road, Salina, KS, 67401, USA
      2. Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, Wisconsin 53706, USA
      3. USDA-ARS National Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333, USA
      4. Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, Ontario, K1A 0C6, Canada
      5. USDA, Agricultural Research Service, Vegetable Crops Research Unit, 1575 Linden Drive, Madison, WI53706, USA
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  • Journal of Genetics | News

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