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      https://www.ias.ac.in/article/fulltext/jgen/097/05/1295-1306

    • Keywords

       

      hybrid purity; GOT; genetic purity; SSR markers; watermelon.

    • Abstract

       

      Genomic simple sequence repeat (SSR) markers were used to fingerprint and determine genetic similarity (GS) of the watermelon breeding lines, as well as the purity of their hybrid derivatives. Cluster analysis and Jaccard’s distance coefficients using the unweighted pair group method with arithmetic mean (UPGMA) have classified these lines into three major groups. Notwithstanding, the genetic background of these lines is narrow as revealed by the restricted GS coefficients. Fifty-five sets of SSR markers were employed in this study. Fourteen of these markers were polymorphic between the breeding lines and were used for assessing hybrid purity. Cross-checking assay validated nine SSR markers as informative SSR markers for purity detection of these hybrids. To confirm the accuracy and efficiency of these markers, their derived PCR products were further sequenced, and ClSSR09643, ClSSR18153 and ClSSR01623 were selected as high-efficiency SSR markers. Interestingly, SSR markers ClSSR09643 and ClSSR18153 were broadly applied for purity detection of more than two different hybrids, while SSR marker ClSSR01623 behaved as a specific marker forpurity detection in this study. Genetic purity of six commercial watermelon hybrids was definitely evaluated using these SSR markers. Genetic purity of all tested hybrids exceeded 96% while the field purity was above 98%. Genetic purity test was an emergency for identifying off-types and selfed female in a lot of hybrid seeds. Here, we elucidated the potential of nine SSR markers including threewith higher breeding selection efficiency. We recommended them to seed company for purity improvement of watermelon commercial hybrid varieties.

    • Author Affiliations

       

      XIA LU1 YAWO MAWUNYO NEVAME ADEDZE1 GILBERT NCHONGBOH CHOFONG2 MAMADOU GANDEKA3 ZHIJUN DENG1 LUHUA TENG1 XUELAI ZHANG1 GANG SUN1 LONGTING SI1 WENHU LI1

      1. Jiangsu Green Port Modern Agriculture Development Company, NanCai Township Road No. 1, Suqian city 223800, People’s Republic of China
      2. Catholic University Institute of Buea, P.O. Box 563, Buea, Cameroon
      3. Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou 350000, People’s Republic of China
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  • Journal of Genetics | News

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