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      https://www.ias.ac.in/article/fulltext/jgen/097/04/0869-0877

    • Keywords

       

      Cobitidae; SoxC; embryogenesis; neurogenesis; gonad development.

    • Abstract

       

      Sox4, a member of the SoxC subfamily which of the Sox family, plays important roles in the development of the vertebrate gonad and nervous system. We have cloned a Sox4 homologue from the brain of Misgurnus anguillicaudatus using homologous cloning and rapid amplification of cDNA ends. We named the cloned gene as MaSox4. The full-length cDNA was 2122 bp, containing a 718bp 5'-untranslated region and a 267 bp 3'-untranslated region. The open-reading frame of the cloned gene encoded 378 amino acids and contained a characteristic HMG-box DNA-binding domain with the specific motif (RPMNAFMVW). Phylogenetic analysis indicated that MaSox4 is highly homologous to Sox4 in different species. Protein sequence analysis showed that MaSox4 is anonsecretory hydrophilic protein. Quantitative real-time reverse transcription polymerase chain reaction and in situ hybridization assay revealed that MaSox4 was ubiquitously expressed during embryogenesis and is present in various adult tissues, especially in the central nervous system. Our study suggests that MaSox4 is highly conserved among vertebrates’ evolution and might be involved in developmental processes such as embryogenesis, neurogenesis and gonad development.

    • Author Affiliations

       

      XIAOHUA XIA1 RUYAN WAN1 WEIRAN HUO1 LINXIA ZHANG1 XIAOPEI XIA1 ZHONGJIE CHANG1

      1. College of Life Science, Henan Normal University, Xinxiang 453007, Henan, People’s Republic of China
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