• MicroRNA-197 induces epithelial–mesenchymal transition and invasion through the downregulation of HIPK2 in lung adenocarcinoma

    • Fulltext

       

        Click here to view fulltext PDF


      Permanent link:
      https://www.ias.ac.in/article/fulltext/jgen/097/01/0047-0053

    • Keywords

       

      lung adenocarcinoma; miR-197; HIPK2; metastasis; epithelial–mesenchymal transition

    • Abstract

       

      The major cause of cancer-related deaths in patients with lung adenocarcinoma (LAD) is due to distant metastasis. Many reports have indicated that miRNA plays a key role in tumour metastasis. The expression of miR-197 is correlated with LADprogression, however, the mechanism of miR-197 is still unknown in the processing of LAD. A Boyden chamber migration/invasion assay was used for the metastatic function study in vitro. Real-time PCR andWestern blot assays were employed to analyse the EMT hallmark changes in both the mRNA and protein levels. 3′-UTR reporter luciferase assay was used to show that HIPK2 is a directtarget of miR-197. miR-197 enhances LAD cell migration and invasion miR-197. The downregulation of miR-197 suppresses the EMT and migration ability. HIPK2 is a direct functional target of miR-197 in LAD metastasis. In summary, miR-197 controls EMT and metastasis by directly silencing HIPK2. The findings suggest that interfering with the miR-197-dependent regulation of HIPK2 could be a useful approach for the treatment of patients with late stage metastatic LAD.

    • Author Affiliations

       

      NAN ZHANG1 LIANG TIAN2 ZHIGANG MIAO2 NAN GUO3

      1. Department ofCardiothoracic Surgery, Cangzhou Central Hospital, Cangzhou 061001, Hebei, People’s Republic of China
      2. Department of Pathology, Cangzhou Central Hospital, Cangzhou 061001, Hebei, People’s Republic of China
      3. Department ofCardiovascular internal medicine, Cangzhou Central Hospital, Cangzhou 061001, Hebei, People’s Republic of China
    • Dates

       
  • Journal of Genetics | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.