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      https://www.ias.ac.in/article/fulltext/jgen/096/06/0941-0950

    • Keywords

       

      pouMU1 gene; sequence mutation; chicken; association analysis; long noncoding RNAs.

    • Abstract

       

      The biological functions of long noncoding RNAs (lncRNAs), which play an important role in regulating development and gene expression, may be affected by variations in lncRNA gene loci or associated genomic sequences. However, the functions of many lncRNAs remain unknown. To analyse correlations between mutations in pouMU1 with chicken growth and carcass traits, 860 chickens from a Gushi×Anka F2 resource population and 96 Lushi, Xichuan, Changshun and recessive white chickens were used to evaluate the genetic effect of the pouMU1 gene. We performed quantitative real-time polymerase chain reaction (qRT-PCR) to analyse the relative expression levels of pouMU1 in nine different tissues and stages of development. pouMU1 expression was highest in pectoralis and leg muscles, whereas no expression was observed in the heart, liver and abdominal fat. Using direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods, two novel sequence mutations(g.1198A>G and g.1238-1239del/insGA) were detected in the pouMU1 gene. SPSS software was used for statistical analysis in association studies. Based on the association data, the presence of both variants was significantly associated with leg muscle fibre width and leg muscle fibre roundness (P < 0.05) and highly associated with leg muscle fibre girth and body weight at 0 week of age (P < 0.01). These data suggest that pouMU1 might participate in regulating chicken muscle development and growth, and the findings offer new insight into the functions of sequence mutations in lncRNAs.

    • Author Affiliations

       

      TUANHUI REN1 2 YANTING ZHOU1 2 YU ZHOU1 2 WEIHUA TIAN1 2 ZHENZHEN GU2 SONG ZHAO1 2 YADI CHEN1 2 RUILI HAN1 2 XIAOJUN LIU1 2 XIANGTAO KANG1 2 ZHUANJIAN LI1 2

      1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, People’s Republic of China
      2. Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou 450002, People’s Republic of China
    • Dates

       
  • Journal of Genetics | News

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