• Distinct classical and molecular cytogenetics of Astyanax marionae and A. fasciatus (Characiformes: Characidae): a comparative study of the organization of heterochromatin and repetitive genes

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      https://www.ias.ac.in/article/fulltext/jgen/096/04/0665-0671

    • Keywords

       

      H3 histone gene; 5S rDNA; C-band; chromosomes; Astyanax fasciatus.

    • Abstract

       

      Genus Astyanax is well distributed in Neotropical freshwater environments and its taxonomic position is uncertain, as is the case with other Characidae genera allocated in the group incertae sedis. This study aimed to analyse the karyotype of different populations of Astyanax fasciatus (Corumbataí River basin) using Giemsa staining, C-band technique, and fluorescence in situ hybridization for the H3 histone and 5S rRNA genes, in addition we describe for the first time the chromosomal organization of H3 histone and 5S rRNAgenes in A. marionae (ParaguayRiver basin). Chromosomes of three A. fasciatus populations were analysed (two with 2n = 50 and one with 2n = 48) and the heterochromatin was organized in two forms (blocks with blurred boundaries and distinct blocks). H3 histone and 5S rRNA genes were observed in all the three populations of A. fasciatus on two chromosome pairs (one metacentric chromosome showing H3 histone and 5S rRNA gene clusters). In A. marionae (2n = 48), H3 histone and5S rRNA genes were observed in one acrocentric chromosome pair (different pairs). Further, differences between karyotypes and heterochromatin, as well as the chromosomal organization of H3 histone and 5S rRNA genes in Astyanax species, focussing on chromosome evolution in the group are discussed.

    • Author Affiliations

       

      DIOVANI PISCOR1 LIANO CENTOFANTE2 PATRICIA PASQUALI PARISE-MALTEMPI1

      1. Laboratório de Citogenética, Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista ‘Júlio de Mesquita Filho’ (UNESP), Av. 24A, 1515, 13506-900, Rio Claro, SP, Brazil
      2. Laboratório de Citogenética e Genética Animal, Instituto de Biociências, Universidade Federal deMato Grosso (UFMT), Rua Fernando Correa da Costa, 2367, 78060-900, Cuiabá, MT, Brazil
    • Dates

       
  • Journal of Genetics | News

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