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      https://www.ias.ac.in/article/fulltext/jgen/096/04/0633-0639

    • Keywords

       

      GSTM1; GSTT1; GSTP1; chronic myeloid leukaemia; imatinib mesylate; single-nucleotide polymorphism.

    • Abstract

       

      The detoxifying activity of glutathione S-transferases (GST) enzymes not only protect cells from the adverse effects of xenobiotics, but also alters the effectiveness of drugs in cancer cells, resulting in toxicity or drug resistance. In this study, we aimed to evaluate the association of GSTM1, GSTT1 and GSTP1 Ile105Val polymorphisms with treatment response among Malaysian chronic myeloid leukaemia (CML) patients who everyday undergo 400 mg of imatinib mesylate (IM) therapy. Multiplex polymerase chain reaction (multiplex-PCR) was performed to detect GSTM1 and GSTT1 polymorphisms simultaneously and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was conducted to detect the GSTP1 Ile195Val polymorphism. On evaluating the association of the variant genotype with treatment outcome, heterozygous variant (AG) and homozygous variant (GG) of GSTP1 Ile105Val showed significantly a higher risk for the development of resistance to IM withOR: 1.951 (95% CI: 1.186–3.209, P = 0.009) and OR: 3.540 (95% CI: 1.305–9.606, P = 0.013), respectively. Likewise, GSTT1 null genotype was also associated with a significantly higher risk for the development of resistance to IM with OR = 1.664 (95% CI: 1.011–2.739, P = 0.045). Our results indicate the potential usefulness of GST polymorphism genotyping in predicting the IMtreatment response among CML patients.

    • Author Affiliations

       

      SITI MAZIRAS MAKHTAR1 AZLAN HUSIN2 ABDUL AZIZ BABA3 RAVINDRAN ANKATHIL1

      1. Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
      2. Haemato-Oncology Unit and Department of Internal Medicine, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
      3. International Medical University, No. 126, Jalan Jalil Perkasa 19, 57000 Bukit Jalil, Kuala Lumpur, Malaysia
    • Dates

       
  • Journal of Genetics | News

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