• The first report of diablo in Megalobrama amblycephala: characterization, phylogenetic analysis, functional annotation and expression

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      https://www.ias.ac.in/article/fulltext/jgen/096/04/0613-0623

    • Keywords

       

      Diablo; physicochemical characterization; homology modelling; functional annotation; gene expression; Megalobrama amblycephala.

    • Abstract

       

      Smac/DIABLO gene is essential for the apoptosis mechanism in mammals. This study is the first report of the Megalobrama amblycephala (ma) diablo gene, and the first report of the tertiary structure of a Diablo polypeptide in fish. Madiablois 1540-bp long with an open reading frame of 792 bp, encoding a putative protein of 263 amino acids with a molecular weight of 29.2 kDa. Phylogenetic analysis indicates that it is closely related to the zebrafish Diablo-a homologue. It also indicates the existence of two diablo copies (a and b) in teleosts; apart fromthe Percomorpha group,where diablo-b has been lost, but diablo-a had undergone an independent duplication. Madiablo protein contains a long Smac_DIABLO super family domain (Leu32–Asp263) and alpha heliceswere prevalent in the secondary structure. Homology model of madiablo protein was constructed using the comparative modelling method. Expression of madiablo mRNA transcript was investigated using qPCR: (i) in five tissues from a healthy blunt snout bream, indicating the highest constitutive expression level in liver. (ii) During the embryo and juvenile development, indicating a spike inexpression during hatching and in later phases of the juvenile development. (iii) In response to Aeromonas hydrophila infection, indicating the downregulation in liver, spleen and kidney during the first 12 h postinfection and upregulation in spleen and kidney after 24 h postinfection (hpi). The results imply that madiablo is homologous to Diablo orthologues in other species, both structurallyand functionally, and that, it probably plays a role in the immune system of M. amblycephala.

    • Author Affiliations

       

      NGOC TUAN TRAN1 2 IVAN JAKOVLIĆ1 WEI-MIN WANG1 3

      1. Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, People’s Republic of China
      2. KeyLaboratory ofAquaculture DiseaseControl, Ministry of Agriculture, and StateKeyLaboratory of FreshwaterEcology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People’s Republic of China
      3. Collaborative Innovation Center for Efficient and Health Production of Fisheries, Changde 41500, Hunan Province, People’s Republic of China
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