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      https://www.ias.ac.in/article/fulltext/jgen/096/04/0571-0582

    • Keywords

       

      rice; salinity tolerance; SNP markers; recombinant inbred line; quantitative trait locus.

    • Abstract

       

      Salinity is the second most important abiotic stress after drought that hampers rice production, especially in south and Southeast Asia. Breeding approach supplemented with molecular markers-assisted selection is the most promising approach in terms of efficiency to increase the productivity under salt-affected soils. Thirty-day-old rice seedlings of 300 F5:6 recombinant-inbred lines derived from a cross between the salt sensitive, IR29 (indica), and a salt tolerant, Hasawi (aus), were used to identify quantitative trait loci (QTLs) linked to salinity tolerance. One hundred and ninety four polymorphic SNP markers were used to construct a genetic linkage map involving 142 selected RILs that covered 1441.96 cM genome with an average distance of 7.88 cM between loci. Twentynew QTLs (LOD > 3) were identified on chromosomes 1, 2, 4, 6, 8, 9 and 12 using composite interval mapping with R2 as high as >20% with LODvalue of 7.21. Many earlier studies reported big qSaltol for seedling stage salinity tolerance in rice is on short arm of chromosome 1 but none of the QTL in our study was on qSaltol or nearby position, therefore, Hasawi conferred salinity tolerance in RILs due to novel QTLs. It is suggested to fine map the novel QTLs so that the level of salinity tolerance could be further enhanced by pyramiding of the different QTLs in one genetic background through marker-assisted selection.

    • Author Affiliations

       

      J. B. BIZIMANA1 2 A. LUZI-KIHUPI1 ROSEMARY W. MURORI3 R. K. SINGH3 4

      1. Department of Crop Science and Production, Plant Breeding, Sokoine University of Agriculture, P.O. Box 3005, Morogoro, Tanzania
      2. Present address: Agricultural Faculty of Sciences (FACAGRO), Department of Crop Production, University of Burundi, P.O. Box 5132, Bujumbura, Burundi
      3. Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute (IRRI), P.O. Box 33581, Dar-Es-Salaam, Tanzania
      4. Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines
    • Dates

       
  • Journal of Genetics | News

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