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      https://www.ias.ac.in/article/fulltext/jgen/096/04/0535-0544

    • Keywords

       

      polymorphisms; Wnt8A gene; growth traits; Qinchuan cattle.

    • Abstract

       

      WNT-ß-catenin-TCF pathway is involved in carcinogenesis and foetal development. As a member of the WNT gene family, Wnt8A encodes secreted signalling proteins and responds to many biological processes.However, similar research on the effects of genetic variations of Wnt8A gene on growth traits is lacking. Therefore, in this study, polymorphisms of Wnt8A were detectedin 396 animals from Chinese Qinchuan cattle using DNA pool sequencing and PCR-RFLP methods. Four novel single-nucleotide polymorphisms (SNPs) of Wnt8A gene were identified, including three mutations in introns (g.T-445C, g.G244C and g.G910A) and one in exon (g.T4922C). Additionally, we examined the associations of four SNPs with growth traits. The results revealed thatSNP2 (g.G244C) was significantly associated with shoulder height, hip height, body length, hip width, and body weight (P <0.05). SNP3 (g.G910A) also displayed notable effects on hip width (P < 0.05). Meanwhile, the haplotype combination CC-GC-GA-CC was strongly associated with heavier, taller and longer animals (P < 0.05). These results show that the Wnt8A gene may be apotential candidate gene, and the SNPs could be used as molecular markers in early marker-assisted selection in beef cattle breeding programmes.

    • Author Affiliations

       

      YONG-ZHEN HUANG1 YONG ZOU1 2 QING LIN2 HUA HE1 2 LI ZHENG3 ZI-JING ZHANG4 YONG-LONG DANG1 CHU-ZHAO LEI1 XIAN-YONG LAN1 XING-SHAN QI5 HONG CHEN1

      1. College of Animal Science and Technology, Northwest A&F University,Yangling, Shaanxi 712100, People’s Republic of China
      2. College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, People’s Republic of China
      3. Henan University of Animal Husbandry and Economy, Zhengzhou, Henan 450046, People’s Republic of China
      4. Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, People’s Republic of China
      5. Bureau of Animal Husbandry, Biyang, Henan 463700, People’s Republic of China
    • Dates

       
  • Journal of Genetics | News

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