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      https://www.ias.ac.in/article/fulltext/jgen/096/02/0341-0351

    • Keywords

       

      FSHR gene; variant transcripts; gene expression; muscovy duck; egg production traits.

    • Abstract

       

      Follicle-stimulating hormone (FSH) and its receptor play a key role in the follicular development and regulation of steroidogenesis in the ovary and spermatogenesis in the testis. The purpose of this study was to characterize themuscovy duck FSHR gene, identify SNPs and their association with egg production traits in muscovy ducks. Here, we cloned the complementary DNA (cDNA) sequence of FSHR, and examined the expression patterns of FSHR gene in adult female muscovy duck tissues. The cloned cDNA of the muscovy duck FSHR gene shared high similarity to those of pekin duck (Anas platyrhynchos) (95.7%) and chicken (93.2%). Three different muscovy duck FSHR transcripts were identified. Quantitative real-time PCR (RT-qPCR) results showed that the FSHR gene was expressed in all the 14 testedtissues, and the highest expression level was seen in the ovary. A total of 16 SNPs were identified, among which, four SNPs were located in the coding region of FSHR. The SNP C320T is significantly associated with egg production at 59 weeks of age (P < 0.05), whereas the SNP A227G is significantly associated with age at first egg stage (P < 0.05).These results suggest that the two SNPs (A227G and C320T) of FSHR gene are associated with egg production traits and could be potential markers that can be used for marker-assisted selection programmes to increase egg production in muscovy duck.

    • Author Affiliations

       

      JIGUO XU1 2 XINFENG GAO1 2 XING LI1 2 QIAO YE1 2 ENDASHAW JEBESSA1 2 BAHARELDIN ALI ABDALLA1 2 QINGHUA NIE1 2

      1. National-Local Joint Engineering Research Center for Livestock Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, People’s Republic of China
      2. Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou 510642, Guangdong, People’s Republic of China
    • Dates

       
  • Journal of Genetics | News

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