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      https://www.ias.ac.in/article/fulltext/jgen/096/02/0273-0281

    • Keywords

       

      reference gene selection; validation; OfTUB; OfRP2; Osmanthus fragrans.

    • Abstract

       

      Accurate normalized data is a primary requisite for quantifying gene expression using RT-qPCR technology. Despite this importance, however, suitable reference genes in Osmanthus fragrans are not available. In this study, seven potentialcandidate reference genes (OfL25-1, OfL25-10, OfRP2, OfTUA, OfTUB3, OfUBQ2 and Of18S) were evaluated to determine which one would be the most reliable reference genes. The expression levels of the candidate referencegenes were analysed by RT-qPCR in flower, leaf, pedicel, blossom bud tissues, as well as in floral organs at different developmental stages.GeNormand NormFinderwere used to statistically analyse transcript variation.Results indicated that OfRP2 and OfL25-10 were the optimal reference genes for use in RT-qPCR when analysing different stages of floral development; while OfTUB3 and OfL25-1 were optimal across tissues. The selected reference genes were used to examineOfMYB1 expression. The results appeared to be useful for future gene expression analyses aiming to characterize developmental stages and tissues of O. fragrans.

    • Author Affiliations

       

      HONGNA MU1 2 TAOZE SUN2 CHEN XU1 LIANGGUI WANG1 YUANZHENG YUE1 HUOGEN LI3 XIULIAN YANG1

      1. College of Landscape Architecture, Nanjing Forestry University, No. 159, Longpan Road, Nanjing, Jiangsu 210037, People’s Republic of China
      2. College of Horticulture and Gardening, Yangtze University, No. 88 Jingmi Road, Jingzhou 434025, Hubei, People’s Republic of China
      3. The Key Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University, No. 159, Longpan Road, Nanjing, Jiangsu 210037, People’s Republic of China
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    • Supplementary Material

       
  • Journal of Genetics | News

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