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      https://www.ias.ac.in/article/fulltext/jgen/096/01/0075-0085

    • Keywords

       

      pig; next-generation sequencing; AhR gene; ARNT gene; single-nucleotide variations; fluorescence in situ hybridization.

    • Abstract

       

      Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor best known for mediating xenobiotic-induced toxicity. AhR requires aryl hydrocarbon receptor nuclear translocator (ARNT) to form an active transcription complex and promote the activation of genes which have dioxin responsive element in their regulatory regions. The present study was performed to determine the complete cDNA sequences of porcine AhR and ARNT genes and their chromosomal localization. Total RNA from porcine livers were used to obtain the sequence of the entire porcine transcriptome by next-generation sequencing (NGS;lllumina HiSeq2500). In addition, both, in silico analysis and fluorescence in situ hybridization (FISH) were used to determine chromosomal localization of porcine AhR and ARNT genes. In silico analysis of nucleotide sequences showed that there were two transcript variants of AhR and ARNT genes in the pig. In addition, computer analysis revealed that AhR gene in the pig is located on chromosome 9 and ARNT on chromosome 4. The results of FISH experiment confirmed the localization of porcine AhR and ARNT genes. In the present study, for the first time, the full cDNAs of AhR and ARNT were demonstrated in the pig.In future, it would be interesting to determine the tissue distribution of AhR and ARNT transcript variants in the pig and to test whether these variants are associated with different biological functions and/or different activation pathways.

    • Author Affiliations

       

      AGNIESZKA SADOWSKA1 LUKASZ PAUKSZTO2 ANNA NYNCA3 IZABELA SZCZERBAL4 KARINA ORLOWSKA1 SYLWIA SWIGONSKA3 MONIKA RUSZKOWSKA1 TOMASZ MOLCAN1 JAN P. JASTRZEBSKI2 GRZEGORZ PANASIEWICZ1 RENATA E. CIERESZKO1 3

      1. Department of Animal Physiology,University of Warmia and Mazury, Oczapowskiego 1A, 10-719 Olsztyn, Poland
      2. Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-719 Olsztyn, Poland
      3. Laboratory of Molecular Diagnostics, University of Warmia and Mazury, Prawochenskiego 5, 10-720 Olsztyn, Poland
      4. Department of Genetics and Animal Breeding, Poznan University of Life Science, Wolynska 33, 60-637 Poznan, Poland
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    • Supplementary Material

       
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