Grain protein content (GPC) and flour whiteness degree (FWD) are important qualitative traits in common wheat. Quantitativetrait locus (QTL) mapping for GPC and FWD was conducted using a set of 131 recombinant-inbred lines derived fromthe cross ‘Chuan 35050
×Shannong 483’ in six environmental conditions. A total of 22 putative QTLs (nine GPC and13 FWD) were identified on 12 chromosomes with individual QTL explaining 4.5–34.0% phenotypic variation. Nine QTLs(40.9%) were detected in two or more environments. The colocated QTLs were on chromosomes 1B and 4B. Among theQTLs identified for GPC,QGpc.sdau-4Afrom the parent Shannong 483 represented some important favourable QTL alleles.QGpc.sdau-2A.1andQFwd.sdau-2A.1had a significant association with both GPC and FWD. The markers detected on topof QTL regions could be potential targets for marker-assisted selection.